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[PSA] your bac water is fine, your fridge door is not

PSA Clean Column ×8 Sourced ×1

your bac water is fine, your fridge door is not. That is the post. The justification is underneath.

Asked SGN what volume they reconstitute to for their own stability testing and they answered in one line. Useful thing to know.

Started reconstituting everything to a round concentration and the six-in-the-morning error rate went to zero.

The dead space question sent me down a rabbit hole for a week. It is a fraction of a unit and it is the same every draw.

I will update this if the picture changes rather than quietly leaving it up.

1,257 up / 179 down88% upvoted60 commentsid klly1326 Jul 2026

60 comments

26 in this archive, depth 5

best — the order this archive was captured in

u/yusuf_achebe97 points·2 days ago

Research-use-only material is not approved for human use, so treat everything in this thread as arithmetic about solutions rather than instructions for a person.

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u/clara_weiss46 points·2 days ago

If your calculation produces a fraction of a unit, the concentration is wrong for your dose. Change the water volume until the numbers land on whole units.

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u/signe_grimaldi0 points·2 days ago

If your calculation produces a fraction of a unit, the concentration is wrong for your dose.

Right, and the arithmetic behind it: 5mg in 1mL is 5mg/mL, so 5mg is 100 units.

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u/soren_zamora44 points·2 days ago

Research-use-only material is not approved for human use, so treat everything in this thread as arithmetic about solutions rather than instructions fo

Disagreeing with this line only: adding more water is not a safety margin, it is a different divisor.

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u/katrin_marchettiOP26 points·2 days ago

Disagreeing with this line only: adding more water is not a safety margin, it is a different divisor.

soren_zamora has the units point exactly right. They are a volume on the barrel and nothing else.

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u/kenji_kimani13 points·2 days ago

The lyophilised cake displaces a small volume, so the true concentration is very slightly lower than the arithmetic suggests. It is a fraction of a percent and it is dwarfed by fill tolerance.

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u/viktor_erdogan10 points·1 days ago

Careful — that arithmetic is wrong. 15mg in 2mL is 7.5mg/mL, so 0.75mg draws to 10 units, not what you wrote.

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u/swirl_dont_shakereconstitution48 points·2 days ago

10mg into 3mL is 3.33mg/mL, and 2.5mg of that is 75 units

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u/viktor_erdogan36 points·1 days ago

The arithmetic in full: concentration is mass over volume, so 15mg into 1mL gives 15mg/mL. A U-100 syringe is graduated at 100 units per millilitre, so 0.75mg is 0.75 divided by 15 millilitres, which is 5 units.

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u/katrin_marchettiOP16 points·1 days ago

I keep a laminated card in the drawer with the arithmetic on it. Looks ridiculous. Has never once let me draw the wrong volume.

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u/clara_weiss8 points·1 days ago

Agreed on the swirl. There is no reason to shake it and a good reason not to.

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u/ines_ekstrom2 points·23 hr ago

Bacteriostatic water contains benzyl alcohol as a preservative, which is what makes repeated entry into the same vial reasonable. Plain sterile water has no preservative.

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u/quarter_dose_queenlow and slow2 points·21 hr ago

I would not round that figure. At 5mg/mL a rounding error of two units is a real fraction of the intended amount.

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u/a1c_arcT2D5 points·1 days ago

I keep a laminated card in the drawer with the arithmetic on it.

Adding one thing — put the date on the label too. Concentration plus date and you can never mix two vials up.

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u/ismael_nwosu0 points·16 hr ago

Adding one thing — put the date on the label too.

This is the sentence that makes the rest unnecessary. Write it on the vial and the problem is gone.

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u/hedda_ekstrom2 points·1 days ago

a U-100 syringe reads 100 units to the millilitre, every time

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u/ahmed_rasmussen1 point·20 hr ago

aim the stream at the glass wall, not at the cake

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u/quiet_reader_9928 points·2 days ago

Wrote the date on the vial as well as the concentration after losing track of which one was opened first.

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[deleted]10 points·2 days ago

[deleted]

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u/rosa_mensah6 points·2 days ago

reconstituting at a round concentration makes every later dose easy

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u/katrin_marchetti-29 points·2 days ago

more water is not more drug, it is the same drug in a bigger volume

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u/vito_beaulieu18 points·1 days ago

Shook the first vial because nobody told me not to. It was fine, but I have swirled every one since.

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u/niels_roos15 points·1 days ago

if the maths gives you a fraction of a unit, change the concentration

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u/ines_ekstrom13 points·23 hr ago

do the arithmetic before you reconstitute, not while holding a syringe

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u/hub_opssite staff8 points·1 days ago

Small fix — a U-100 syringe is 100 units per mL. If yours is marked differently it is not U-100 and the whole calculation changes.

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u/santiago_ndiaye4 points·20 hr ago

Agreed — units are volume. Once that lands, the rest of the arithmetic is one division.

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About c/reconstitution

The arithmetic and the technique. Bacteriostatic versus sterile water, reconstitution volume as a concentration choice, converting mg/mL into insulin-syringe units, dead space, swirl-not-shake, stopper coring, and how long a reconstituted vial is actually good for. Math errors get corrected fast and politely.

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