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[Question] does needle dead space actually matter at 2.5mg doses

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Question in the title, detail here: does needle dead space actually matter at 2.5mg doses.

Numbers, in the order they matter: 2.5mg.

I keep a laminated card in the drawer with the arithmetic on it. Looks ridiculous. Has never once let me draw the wrong volume.

Spent 22 weeks confused about this until somebody drew the table for me. 5mg, 1mL, 5mg/mL, 2mg is 40 units. That is the whole thing.

If somebody has the same thing measured a different way, post it next to mine and we will see whether they agree.

464 up / 98 down83% upvoted30 commentsid k1mqn525 Jul 2026

30 comments

21 in this archive, depth 5

best — the order this archive was captured in

u/tl_dr_tina50 points·3 days ago

Why "more water" keeps confusing people, including me for a month.

The vial contains a fixed amount of peptide. Water changes nothing about that amount. What it changes is concentration, and concentration is the divisor between the dose you want and the volume you draw.

So doubling the water halves the concentration and doubles the units for the same dose. Nothing is diluted in any sense that matters; you are simply reading a bigger number off the same barrel. Pick the water volume that puts your usual dose on a whole number of units and life gets easier.

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u/units_not_mgMOD43 points·4 days ago

Reminder: this board does maths, not dosing. Nobody here tells another member what amount to take.

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u/oskar_kaufmann34 points·4 days ago·edited

Bacteriostatic or plain sterile water?

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u/saskia_mwangi17 points·4 days ago

the powder cake sits at the bottom, the volume you add is the volume that counts

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u/niels_roos14 points·4 days ago·edited

Small fix — a U-100 syringe is 100 units per mL. If yours is marked differently it is not U-100 and the whole calculation changes.

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[deleted]10 points·4 days ago

[deleted]

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u/gradient_goblin9 points·4 days ago

Agreed on the swirl. There is no reason to shake it and a good reason not to.

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u/quiet_reader_9921 points·4 days ago·edited

Is that a U-100 syringe? The barrel markings change the answer completely.

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u/georgi_adebayo13 points·3 days ago

reconstituting at a round concentration makes every later dose easy

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u/lyophile_lou27 points·4 days ago

Show the arithmetic — where did the unit figure come from?

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u/adaeze_cabrera23 points·3 days ago

The arithmetic in full: concentration is mass over volume, so 30mg into 3mL gives 10mg/mL. A U-100 syringe is graduated at 100 units per millilitre, so 5mg is 5 divided by 10 millilitres, which is 50 units.

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u/oskar_ibarra11 points·3 days ago

What is written on the vial right now?

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u/rina_bergstrom7 points·3 days ago

Adding more water does not change how much peptide is in the vial. It changes the concentration, which changes the volume you draw for the same amount. That is the entire relationship.

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u/mateusz_achebe19 points·3 days ago

Shook the first vial because nobody told me not to. It was fine, but I have swirled every one since.

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u/hedda_ekstrom11 points·2 days ago·edited

Shook the first vial because nobody told me not to.

mateusz_achebe has the units point exactly right. They are a volume on the barrel and nothing else.

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u/gradient_goblin12 points·4 days ago

Started reconstituting everything to a round concentration and the six-in-the-morning error rate went to zero.

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u/priya_guerrero4 points·4 days ago

I would not round that figure. At 6.67mg/mL a rounding error of two units is a real fraction of the intended amount.

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u/aurel_boateng4 points·4 days ago

Asked JEEP what volume they reconstitute to for their own stability testing and they answered in one line. Useful thing to know.

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u/emil_zamora6 points·4 days ago·edited

How many mg in the vial and how many mL are you adding?

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u/emil_wojcik9 points·3 days ago

What concentration did you actually reconstitute at, rather than what did you intend to?

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u/sock_puppet_spotterOP7 points·3 days ago

30mg into 3mL is 10mg/mL, and 2.5mg of that is 25 units

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