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Single comment threadYou are looking at one branch of genuine question about HPLC that I am slightly embarrassed to ask — 46 comments in the full submission. View in context.
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c/hplc·submitted 1 year ago by u/bilal_adebayo

genuine question about HPLC that I am slightly embarrassed to ask

Discussionbranch of 8 comments

Question in the title, detail here: genuine question about HPLC that I am slightly embarrassed to ask. MKM sent me the column, the gradient and the theoretical mass without being asked. That is a short list and they are on it. Started reporting my own integrations with the baseline choice stated. Arguments in my…

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8 comments, started 1 year ago
u/priya_ogunleye192 points·1 year ago

Push back: a longer run is not automatically better resolution. You are trading peak width for time and the ratio is what matters.

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u/b12_baseline93 points·1 year ago

integration decisions move the number more than the sample does

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u/cormac_roos-14 points·1 year ago

the column has a history and it shows in the peak shape

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u/sig_figs_sammod · analytical1 point·1 year ago

report the method or do not report the number

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u/saskia_bakker1 point·1 year ago

214nm sees the peptide bond, 280nm sees the aromatics

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u/step_count_stan1 point·1 year ago

retention time alone is not identity

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u/ignacio_vanhecke154 points·1 year ago

baseline choice is a decision, not a measurement

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u/priya_ogunleye81 points·1 year ago

Cosigning the wavelength point. Half the disagreements in this board are two people looking at different detectors.

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