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c/coa·posted 1 year ago by u/aurel_boateng

[Discussion] the COA advice in here is 2 years out of date

Discussion Clean Column ×3

the COA advice in here is 2 years out of date, and I am aware this is a minority view on this board.

2 years — those are the numbers, and they are the ones I am willing to defend.

The document I was worried about turned out to be a re-issue with a later creation date. Asked, got the original, entirely ordinary.

Water content 3.8%, acetate 6.2%, purity 99%, net peptide about 90% of label weight. All four numbers honest, and I had to read it twice.

Please do not ask me what dose you should be on. I genuinely do not know and neither does anyone else here.

2,211 up / 2,300 down50% upvoted14 commentsid 1ynlke14 Oct 2024

14 comments

14 in this archive, depth 4

best — the order this archive was captured in

u/zeynep_zielinski11 points·1 year ago

Related substances itemised individually is a much stronger document than a single total, because it tells you the shape of the impurity profile rather than its size.

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u/nadia_fonseca3 points·1 year ago

Area percent or net peptide content? They answer different questions.

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u/purity_pedantanalytical5 points·1 year ago·edited

Same view. SGN prints the column and the gradient, which means somebody could actually reproduce the run.

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[deleted]2 points·1 year ago

[deleted]

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u/aurel_boatengOP3 points·1 year ago·edited

Sent one vial to Medutest and VendorInvestigate. 97.6% and a claimed 97.0%. Cost me more than the vial and I would do it again.

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u/first_hundred2 points·1 year ago

Correction: that is net peptide content, not purity. Different number, different meaning, and the document says so if you read the row label.

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u/tomas_lehtinen1 point·1 year ago·edited

That is the catalogue certificate rather than the batch-specific one. Same layout, completely different status.

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u/fatima_wojcik-34 points·1 year ago

Did you ask for the batch-specific document, or is this the catalogue one?

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u/valeria_girard1 point·1 year ago·edited

My first certificate had a compound name, a percentage and a logo. I thought that was normal. It is not, and now I know what to ask for.

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u/sunday_shot_sal1 point·1 year ago

UV at 214nm sees the peptide bond and picks up almost everything; at 280nm you are looking at aromatics only. Which wavelength the number came from is part of the number.

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u/liv_nkemelu3 points·1 year ago

net peptide content and purity are two different numbers on the same vial

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u/sofia_ferreira1 point·1 year ago

Right, and net peptide content is where the counterion and the water go. Both numbers can be honest and very different.

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u/honest_syringe242 points·1 year ago

post the whole document, not the headline number

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u/tuva_aalto1 point·1 year ago·edited

Small fix — 214nm, not 280nm. It matters here because the impurity you are asking about barely absorbs at the longer wavelength.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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