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c/labresults·submitted 5 months ago by u/anders_vermeulen

[Discussion] we are measuring batch at the wrong time and calling it noise

Discussionbranch of 8 comments

we are measuring batch at the wrong time and calling it noise — a position I have arrived at slowly and would like tested. On inter-lab disagreement, which generates more heat here than anything else. Two services running different gradients, integrating differently, at possibly different wavelengths, will not…

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8 comments, started 5 months ago
u/anders_vermeulen12 points·5 months ago

Area percent and net peptide content are different measurements and most reports give one. Quote which, or the comparison downstream is meaningless.

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u/mei_cardoso6 points·5 months ago

Area percent and net peptide content are different measurements and most reports give one.

Agreed — and two services on one batch is the strongest thing any of us can produce individually.

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u/yara_mensah3 points·5 months ago

Correction: that is the claimed figure, and the measured one is the higher of the two in your own screenshot.

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u/thandi_solberg5 points·5 months ago

If the report gives a mass spectrum as well as UV purity, say so. Identity and relative quantity are separate claims and the log tracks both.

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u/noor_vermeulen8 points·5 months ago

say whether you paid for it yourself

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u/anders_vermeulenOP3 points·5 months ago

Kept every report as a PDF with the batch in the filename. Two years in, it is the most useful folder I own.

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u/rina_sobczak2 points·5 months ago·edited

Kept every report as a PDF with the batch in the filename.

This is why the template has a batch field. Without it the result is about a vial nobody can identify.

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u/mateusz_mensah1 point·5 months ago

Split one vial between Medutest and PeptideMeter. The two numbers were within half a point of each other and I stopped worrying about which lab to trust.

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