[Meta] proposal — a flair for HPLC posts
proposal — a flair for HPLC posts. This affects how posts get sorted and removed, so it belongs in the open. Chased a ghost peak for three weeks. It was the plumbing. It is always the plumbing. A shoulder that does not baseline-resolve cannot be quantified honestly. You can report it as an unresolved shoulder, which…
retention time alone is not identity
Small fix — 214nm, not 210. It matters for the comparison you are making with the other run.
How old is the column and roughly how many injections has it seen?
How old is the column and roughly how many injections has it seen?
anya_erdogan is right — the integration choice is a decision and it should be stated alongside the result.
integration decisions move the number more than the sample does
Agreed, and it is why Homopeptide printing the column and gradient on the certificate is genuinely useful rather than decorative.
you cannot report to two decimals off that baseline
Can you post the trace with the axes labelled?
Can you post the trace with the axes labelled?
This is the whole methodological point of the board in one line.
Yes — system suitability first. Without it the number is an assertion about the instrument, not the sample.
GL Biochem sent me the column, the gradient and the theoretical mass without being asked. That is a short list and they are on it.