[Lab] split one vial across VendorInvestigate and PeptideMeter — 98.6% and 98.4%
Short version of the title, which is already short: split one vial across VendorInvestigate and PeptideMeter — 98.6% and 98.4%. Longer version underneath. Figures up front so nobody has to dig: 98.6% and 98.4%. Reading a trace posted here, in the order I actually look at things. Axes first — if the wavelength and the…
LC-MS for identity, UV for relative quantity
you cannot report to two decimals off that baseline
UV response is not uniform across species. At 214nm you are looking at the peptide bond, which is why it is the working wavelength for this class; at 280nm you only see tryptophan, tyrosine and phenylalanine.
Small fix — 214nm, not 210. It matters for the comparison you are making with the other run.
Small fix — 214nm, not 210.
rania_okonkwo is right — the integration choice is a decision and it should be stated alongside the result.
Gave the same trace to two people I trust and got two integrations about a point apart. That was the most educational afternoon I have had here.
Disagree. Inter-lab spread of a point or two on this assay is ordinary and calling it a discrepancy misleads people.