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c/coa·submitted 5 months ago by u/certified_reference

unpopular opinion: most of what gets said here about COA is guesswork

Suspiciousbranch of 9 comments

The title is the argument: unpopular opinion: most of what gets said here about COA is guesswork. Here is the rest of it. The PDF creation date being later than the analysis date is usually a re-issue or a re-export. It is worth one question and it is not evidence of anything on its own. Ask what the stability…

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9 comments, started 5 months ago
u/amara_halonen528 points·5 months ago

Related substances itemised individually is a much stronger document than a single total, because it tells you the shape of the impurity profile rather than its size.

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u/santiago_szabo441 points·5 months ago

Area percent is the peak area of your compound over the total integrated area, at one wavelength, on one gradient. It is a relative measure of what the detector saw, not a mass fraction of the vial.

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u/bastian_moreau425 points·5 months ago

This. Area percent answers "what fraction of what the detector saw was the peak I wanted", and people read it as "how much peptide is in the vial".

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u/santiago_szabo108 points·5 months ago·edited

redact your own name, never the batch details

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u/soren_nkemelu53 points·5 months ago

Are the related substances itemised or is it a single total?

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u/certified_referenceOPQC-4 points·5 months ago

Related substances itemised individually is a much stronger document than a single total, because it tells you the shape of the impurity profile rathe

amara_halonen has the distinction right — one is a fraction of the detector signal, the other is a fraction of the mass.

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u/dario_boateng1 point·5 months ago

Yes. The structure of the document matters at least as much as the number printed on it.

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u/tomas_lehtinen1 point·5 months ago

The document I was worried about turned out to be a re-issue with a later creation date. Asked, got the original, entirely ordinary.

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u/joaquin_ivaturi1 point·5 months ago

Cosigning. The batch identifier is load-bearing, and a certificate that cannot be tied to the vial in your hand is not about your vial.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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