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c/coa·submitted 2 years ago by u/sig_figs_sam

[Meta] the batch rule is doing its job and people should stop complaining

COAbranch of 8 comments

the batch rule is doing its job and people should stop complaining. Short post, long comment section, probably. Area percent is the peak area of your compound over the total integrated area, at one wavelength, on one gradient. It is a relative measure of what the detector saw, not a mass fraction of the vial. UV at…

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8 comments, started 2 years ago
u/anders_kuusela187 points·2 years ago·edited

Net peptide content is the fraction of the powder that is actually peptide once counterion, water and residual solvent are accounted for. A 99% pure acetate salt can still be about 85% peptide by mass.

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u/anya_moreau-14 points·2 years ago

the related substances line is where the story is

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u/emeka_chowdhury1 point·2 years ago

That is the catalogue certificate rather than the batch-specific one. Same layout, completely different status.

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u/anya_moreau1 point·2 years ago

identity and quantity are two questions and most documents answer one

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u/sig_figs_samOPmod · analytical0 points·2 years ago

Small fix — 214nm, not 280nm. It matters here because the impurity you are asking about barely absorbs at the longer wavelength.

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u/gastric_emptying_g1 point·2 years ago

Pushing back a little: a missing field is usually presentation, not concealment. Ask for the full document before drawing conclusions.

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u/blunt_coldbox291 point·2 years ago

Pushing back a little: a missing field is usually presentation, not concealment.

Agreed, and the practical version is: ask for the itemised table. If it exists, you get it.

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u/sig_figs_samOPmod · analytical76 points·2 years ago·edited

Does the lot on the vial match the lot on the page?

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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