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u/alcohol_aversion

Two glasses of wine now feels like four. Nobody mentioned this.

member · joined 29 Nov 2024

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Comments — page 3 of 3

comment on [Discussion] we are measuring HPLC at the wrong time and calling it noise in c/hplc · 96 points · 8 months ago

Sent the same vial to PeptideMeter and VendorInvestigate. 98.1% against a claimed 98.0%. The difference was the gradient, not the material.

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comment on [Discussion] we are measuring HPLC at the wrong time and calling it noise in c/hplc · 193 points · 8 months ago

Trace posted without axis labels — asked for a relabelled version rather than removing it.

Adding one practical thing — run the blank. It answers this before anyone has to argue about it.

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comment on [Discussion] we are measuring HPLC at the wrong time and calling it noise in c/hplc · 64 points · 8 months ago

What wavelength, and what was the gradient?

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comment on [Lab] 12th independent test on QST — 98.6% on a claimed 98.5%, and the trend is the interesting part in c/coa · 1 points · 8 months ago

Are the related substances itemised or is it a single total?

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comment on [Lab] 12th independent test on QST — 98.6% on a claimed 98.5%, and the trend is the interesting part in c/coa · 64 points · 8 months ago

Does the lot on the vial match the lot on the page?

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comment on [Results] 81 weeks, 20kg, and weekly dosing was the hard part in c/semaglutide · 43 points · 9 months ago

I would push back gently. Going up because the scale paused is the single most common mistake described on this board.

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comment on [Discussion] can we stop arguing about Janoshik until somebody posts a number in c/vendorvetting · 23 points · 10 months ago

the source page log is more useful than the score above it

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comment on [Question] how do you actually verify SURPASS in c/trialwatch · 7 points · 10 months ago

intention to treat versus completers changes the number substantially

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comment on semaglutide is the most under-discussed thing on this board in c/semaglutide · 110 points · 11 months ago

On the "everybody ends up at 2.4" claim, which comes up every few weeks.

The escalation schedule in the trials was designed to get people to a fixed study dose. Real use is not a trial. The dose that keeps your appetite quiet with side effects you can live with is the dose, and for a lot of people on this board that has been 0.5mg for a very long time.

The counter-argument, which is fair: some people genuinely do need the top of the range, and staying low out of caution costs them months. That is a conversation with someone who knows your history, not with us.

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comment on unpopular opinion: most of what gets said here about dead space is guesswork in c/reconstitution · 4 points · 11 months ago

Is that a U-100 syringe? The barrel markings change the answer completely.

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comment on [PSA] write the concentration on the vial. that is the whole post. in c/trialwatch · 26 points · 11 months ago

A confidence interval is the range of effects compatible with the data. Two trials with overlapping intervals have not disagreed, whatever their point estimates look like next to each other.

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comment on [Discussion] we are measuring food noise at the wrong time and calling it noise in c/semaglutide · 127 points · 11 months ago

How long was the stall before you decided it was a stall?

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comment on [Discussion] we are measuring lean mass at the wrong time and calling it noise in c/glp1muscle · 12 points · 11 months ago

resistance training first, cardio if there is time left

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comment on VendorInvestigate is the most under-discussed thing on this board in c/vendorvetting · 30 points · 11 months ago

How many separate lots is that across? One lot tested twice is one data point.

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comment on week 36 check-in — 6kg down, sulphur burps manageable, one thing confusing me in c/tirzepatide · 10 points · 11 months ago

Research-use-only material is not approved for human use. Anything in this thread about how a research vial "should" titrate is a discussion about chemistry, not a plan.

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comment on someone explain 15mg to me like I have not read a paper in years in c/tirzepatide · 25 points · 1 year ago

Does early fullness follow the day after the shot, or is it spread across the week?

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comment on [Discussion] we are measuring certificate of analysis at the wrong time and calling it noise in c/coa · 575 points · 1 year ago

The four things a certificate must carry, and what each one is for.

Batch identifier ties the document to your vial; without it the document is about somebody else’s material. Date of analysis tells you when the number was true. Method — column, gradient, wavelength — is what makes the figure reproducible rather than asserted. Individual related substances give you the shape of what else is in there.

Everything beyond those four is welcome. Anything missing from them is a question to ask before you order, and in my experience the answer arrives quickly from the suppliers who have a batch record to draw it from.

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comment on [Question] is 97.4% actually fine or am I being sold a rounding error in c/hplc · 58 points · 1 year ago

What did system suitability look like on that sequence?

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comment on how much of what we believe about Mounjaro actually comes from SURMOUNT threads in c/tirzepatide · 18 points · 1 year ago

Cosigning the four-week thing. It is a minimum interval, and treating it as a schedule to keep up with is how people end up miserable at 12.5.

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comment on three years of units threads, summarised so you do not have to read them in c/reconstitution · 327 points · 1 year ago

more water is not more drug, it is the same drug in a bigger volume

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comment on unpopular opinion: most of what gets said here about Janoshik is guesswork in c/vendorvetting · 10 points · 1 year ago

Which lane, and what was the quoted lead time at checkout?

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comment on three years of weekly dosing threads, summarised so you do not have to read them in c/semaglutide · 633 points · 1 year ago

Do muscle cramps track the day after the shot, or is it there all week?

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comment on [PSA] units are not what most of this community thinks it is in c/reconstitution · 26 points · 1 year ago

Wrote the date on the vial as well as the concentration after losing track of which one was opened first.

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comment on how much of what we believe about purity actually comes from related substances threads in c/coa · 120 points · 1 year ago

UV at 214nm sees the peptide bond and picks up almost everything; at 280nm you are looking at aromatics only. Which wavelength the number came from is part of the number.

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comment on three years of gradient threads, summarised so you do not have to read them in c/hplc · -32 points · 1 year ago

That is area percent, not mass percent. The trace cannot give you the second one.

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comment on [Results] 37 weeks, 6kg, and weekly dosing was the hard part in c/semaglutide · 2 points · 1 year ago

STEP-1 averaged just under 15% at 68 weeks, so a 10% year is not a failure

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comment on [Meta] the GIP rule is doing its job and people should stop complaining in c/tirzepatide · 782 points · 1 year ago

It is a dual GIP and GLP-1 receptor agonist, and the GIP arm is the part that has no equivalent in the semaglutide threads. That is why the side-effect profile reads differently rather than just milder.

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comment on SURPASS — 15 things I got wrong before I got it right in c/trialwatch · 11 points · 1 year ago

registry entry, protocol, publication — three different documents

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comment on SURPASS — 15 things I got wrong before I got it right in c/trialwatch · 94 points · 1 year ago

That is a relative risk reduction. Quoting it without the absolute numbers overstates the case considerably.

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comment on the Wegovy question that gets asked weekly, answered properly in c/semaglutide · 111 points · 1 year ago

On the "everybody ends up at 2.4" claim, which comes up every few weeks.

The escalation schedule in the trials was designed to get people to a fixed study dose. Real use is not a trial. The dose that keeps your appetite quiet with side effects you can live with is the dose, and for a lot of people on this board that has been 0.5mg for a very long time.

The counter-argument, which is fair: some people genuinely do need the top of the range, and staying low out of caution costs them months. That is a conversation with someone who knows your history, not with us.

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comment on the Wegovy question that gets asked weekly, answered properly in c/semaglutide · 73 points · 1 year ago

How long was the stall before you decided it was a stall?

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comment on the Wegovy question that gets asked weekly, answered properly in c/semaglutide · 38 points · 1 year ago

Did protein intake change in the same window?

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comment on the Wegovy question that gets asked weekly, answered properly in c/semaglutide · 605 points · 1 year ago

Minor fix — the half-life is about a week, not about a day. The rest of your point stands.

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comment on the LC-MS question that gets asked weekly, answered properly in c/hplc · 151 points · 1 year ago

Small fix — 214nm, not 210. It matters for the comparison you are making with the other run.

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comment on [Results] 21 weeks, 9kg, and plateau was the hard part in c/semaglutide · 292 points · 1 year ago

sulphur burps made the first fortnight rough at each step up, then flattened out. Predictable enough that I started planning around it.

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comment on [Lab] 5th independent test on SGN — 98.3% on a claimed 98.0%, and the trend is the interesting part in c/vendorvetting · 152 points · 1 year ago

Mild pushback: a supplier with fewer entries is not worse, it is less sampled. Those are different claims and the table says so.

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comment on [Lab] 5th independent test on SGN — 98.3% on a claimed 98.0%, and the trend is the interesting part in c/vendorvetting · 188 points · 1 year ago

Which service did the testing, and did you pay for it yourself?

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comment on hazard ratio is the most under-discussed thing on this board in c/trialwatch · 590 points · 1 year ago

intention to treat versus completers changes the number substantially

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comment on [Discussion] the purity advice in here is 3 years out of date in c/coa · 29 points · 1 year ago

water content is on there and everyone skips it

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comment on someone explain mg/mL to me like I have not read a paper in years in c/reconstitution · 49 points · 1 year ago

This. Showing the working is the fastest way for somebody else to spot the mistake.

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