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c/reconstitution·posted 19 days ago by u/signe_grimaldi

reconstituted at 1mL instead of 2mL and caught it before injecting. lucky.

Math Slow Clap ×7 Sourced ×3

reconstituted at 1mL instead of 2mL and caught it before injecting. lucky. It is the sort of thing everyone half-believes and nobody writes down.

The relevant figures are 1mL and 2mL, and they come from the same log I have kept the whole time.

Asked SSA what volume they reconstitute to for their own stability testing and they answered in one line. Useful thing to know.

Why "more water" keeps confusing people, including me for a month.

The vial contains a fixed amount of peptide. Water changes nothing about that amount. What it changes is concentration, and concentration is the divisor between the dose you want and the volume you draw.

So doubling the water halves the concentration and doubles the units for the same dose. Nothing is diluted in any sense that matters; you are simply reading a bigger number off the same barrel. Pick the water volume that puts your usual dose on a whole number of units and life gets easier.

Ask me anything specific. Anything general I will probably get wrong.

2,181 up / 119 down95% upvoted51 commentsid 18uete11 Jul 2026

51 comments

30 in this archive, depth 5

best — the order this archive was captured in

u/andres_sorensen268 points·18 days ago

Adding more water does not change how much peptide is in the vial. It changes the concentration, which changes the volume you draw for the same amount. That is the entire relationship.

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u/signe_grimaldiOP104 points·17 days ago

do the arithmetic before you reconstitute, not while holding a syringe

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u/signe_grimaldiOP64 points·17 days ago

How many mg in the vial and how many mL are you adding?

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u/rasmus_mensah50 points·17 days ago

Careful — that arithmetic is wrong. 5mg in 3mL is 1.67mg/mL, so 0.75mg draws to 45 units, not what you wrote.

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u/kenji_kimani16 points·17 days ago

bacteriostatic water has the preservative, sterile water does not

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u/georgi_adebayo154 points·17 days ago

Research-use-only material is not approved for human use, so treat everything in this thread as arithmetic about solutions rather than instructions for a person.

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u/ledger_modmod · vetting72 points·17 days ago

What is written on the vial right now?

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u/piotr_bruun30 points·17 days ago

Small fix — a U-100 syringe is 100 units per mL. If yours is marked differently it is not U-100 and the whole calculation changes.

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u/rasmus_kuipers30 points·17 days ago

units are a volume on a U-100 syringe, not a dose

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u/quarter_dose_queenlow and slow7 points·17 days ago

Spent 20 weeks confused about this until somebody drew the table for me. 5mg, 3mL, 1.67mg/mL, 1.5mg is 90 units. That is the whole thing.

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u/tomas_kimani2 points·16 days ago·edited

Started reconstituting everything to a round concentration and the six-in-the-morning error rate went to zero.

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u/sleep_apnea_sam124 points·17 days ago·edited

That only works if the fill weight is exactly the label weight, and it usually is not exactly anything.

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u/andres_sorensen0 points·17 days ago

I would not round that figure. At 5mg/mL a rounding error of two units is a real fraction of the intended amount.

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u/emil_okwuosa1 point·17 days ago

I would not round that figure.

This is the sentence that makes the rest unnecessary. Write it on the vial and the problem is gone.

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u/signe_grimaldiOP1 point·17 days ago

Correction: that gives 30 units, not what you have written. 5mg into 2mL is 2.5mg/mL and the division follows from there.

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u/hedda_adeyemi1 point·17 days ago

swirl, never shake

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u/yusuf_nakamura78 points·17 days ago

The lyophilised cake displaces a small volume, so the true concentration is very slightly lower than the arithmetic suggests. It is a fraction of a percent and it is dwarfed by fill tolerance.

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u/marisol_frisk50 points·17 days ago

aim the stream at the glass wall, not at the cake

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u/emeka_delgado43 points·17 days ago

write the concentration on the vial in marker, that is the whole post

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u/bac_water_banditMOD24 points·17 days ago

Reminder: this board does maths, not dosing. Nobody here tells another member what amount to take.

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u/RetaAndRegret211 points·17 days ago

the number you write down is the number you will trust at 6am

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u/viktor_erdogan7 points·17 days ago

Reconstituted at 2mL when I meant to use a different volume. Caught it because the units looked wrong on the barrel. Now I write 10mg/mL on the glass before I put the cap back.

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u/spain_receta33 points·18 days ago·edited

The arithmetic in full: concentration is mass over volume, so 30mg into 2mL gives 15mg/mL. A U-100 syringe is graduated at 100 units per millilitre, so 1.5mg is 1.5 divided by 15 millilitres, which is 10 units.

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u/nadia_kjaer18 points·18 days ago

That is sterile water, not bacteriostatic. Same appearance, different product, and it matters for a multi-draw vial.

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u/anya_antonsen21 points·18 days ago

if the maths gives you a fraction of a unit, change the concentration

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u/signe_grimaldi5 points·18 days ago·edited

Bacteriostatic or plain sterile water?

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u/viktor_erdogan28 points·17 days ago

Show the arithmetic — where did the unit figure come from?

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[removed]21 points·17 days ago

[removed by moderator]

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u/quarter_dose_queenlow and slow16 points·17 days ago

Is that a U-100 syringe? The barrel markings change the answer completely.

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u/sigrid_kaufmann17 points·16 days ago

Not quite. Bacteriostatic and sterile water are not interchangeable for a multi-draw vial, and the difference is the preservative.

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About c/reconstitution

The arithmetic and the technique. Bacteriostatic versus sterile water, reconstitution volume as a concentration choice, converting mg/mL into insulin-syringe units, dead space, swirl-not-shake, stopper coring, and how long a reconstituted vial is actually good for. Math errors get corrected fast and politely.

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