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c/hplc·submitted 1 year ago by u/rina_bergstrom

[Question] how do you actually verify HPLC

Questionbranch of 6 comments

how do you actually verify HPLC. Searched first, found three threads that contradict each other, hence the post. Ran a blank after a high-concentration injection and found the carryover I had been calling an impurity. Retention time shifted 0.4 minutes and I assumed the worst. It was the column temperature. Bought a…

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6 comments, started 1 year ago
u/anya_erdogan19 points·1 year ago

reproducibility beats resolution if you only get one of them

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u/rina_bergstromOP-6 points·1 year ago

Can you post the trace with the axes labelled?

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u/rina_bergstromOP1 point·1 year ago

Is that baseline drawn by the software or by hand?

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u/reverse_image_ron1 point·1 year ago·edited

Correction: that is a tailing factor, not a plate count. Different diagnostics for different problems.

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u/hazard_ratio_halstats1 point·1 year ago

This. A shoulder that does not baseline-resolve is a question, not a quantity.

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u/dilara_nyberg1 point·1 year ago

a shoulder is not an impurity until you can resolve it

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