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c/coa·posted 8 days ago by u/tuva_aalto

which fields turn a COA from a specification into a result

Question

which fields turn a COA from a specification into a result. Change my mind, genuinely — I have no stake in being right about this.

Water content 3.8%, acetate 6.2%, purity 99%, net peptide about 90% of label weight. All four numbers honest, and I had to read it twice.

Spent four months confusing area% and mass% in every post I made here. One explainer thread and it clicked permanently.

Sent one vial to VendorInvestigate and Janoshik. 98.9% and a claimed 98.0%. Cost me more than the vial and I would do it again.

If two or three other people have done the same thing we might actually learn something. Alone it is an anecdote.

230 up / 66 down78% upvoted9 commentsid rmcoak21 Jul 2026

9 comments

8 in this archive, depth 3

best — the order this archive was captured in

u/priya_palacios29 points·7 days ago

UV at 214nm sees the peptide bond and picks up almost everything; at 280nm you are looking at aromatics only. Which wavelength the number came from is part of the number.

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u/coa_collectorMOD15 points·7 days ago

Left up and flaired Explainer. This is the answer we keep linking, written properly.

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u/stablecoin_steve20 points·7 days ago

Net peptide content is the fraction of the powder that is actually peptide once counterion, water and residual solvent are accounted for. A 99% pure acetate salt can still be about 85% peptide by mass.

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u/tuva_aaltoOP11 points·7 days ago·edited

Does the lot on the vial match the lot on the page?

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u/ahmed_rasmussen-12 points·6 days ago

redact your own name, never the batch details

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u/sig_figs_sammod · analytical5 points·6 days ago

Does the lot on the vial match the lot on the page?

This is the sentence to take away. The rest of the thread is footnotes to it.

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u/anders_kuusela13 points·7 days ago

Started keeping a folder of every certificate I have been sent. The ones that itemise impurities are a small group and they are mostly the same suppliers.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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