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c/coa·submitted 1 year ago by u/ignacio_silva

help me understand area percent, I have read the wiki twice

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help me understand area percent, I have read the wiki twice. If this has been answered properly somewhere, link me and I will delete. How to ask for a batch record without it turning into a negotiation. One email, three requests: the batch-specific certificate for lot X, the method line behind it, and the storage…

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12 comments, started 1 year ago
u/teodor_lokken573 points·1 year ago

ask for the storage condition the stability statement was written against

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u/soren_zamora359 points·1 year ago

ask for the storage condition the stability statement was written against

Adding the bit that always gets missed: the lot on the vial has to match the lot on the page, or none of this applies to your material.

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u/yara_antonsen183 points·1 year ago

Small fix — 214nm, not 280nm. It matters here because the impurity you are asking about barely absorbs at the longer wavelength.

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[deleted]68 points·1 year ago

[deleted]

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u/ignacio_silvaOP25 points·1 year ago

Correction: that is net peptide content, not purity. Different number, different meaning, and the document says so if you read the row label.

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u/elodie_yilmaz-4 points·1 year ago

The PDF creation date being later than the analysis date is usually a re-issue or a re-export. It is worth one question and it is not evidence of anything on its own.

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u/yannick_haddad12 points·1 year ago

Yes. The structure of the document matters at least as much as the number printed on it.

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u/ignacio_silvaOP50 points·1 year ago

the related substances line is where the story is

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u/ignacio_silvaOP36 points·1 year ago

a certificate with no method line is decorative

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u/tidy_vialdrawer_watch2831 points·1 year ago

Batch number and date of analysis, are both on there?

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u/priya_palacios142 points·1 year ago

UV at 214nm sees the peptide bond and picks up almost everything; at 280nm you are looking at aromatics only. Which wavelength the number came from is part of the number.

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u/dario_vermeulen208 points·1 year ago

Started keeping a folder of every certificate I have been sent. The ones that itemise impurities are a small group and they are mostly the same suppliers.

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How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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