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c/coa·posted 2 years ago by u/zeynep_zielinski

[Vendor] TFC vs SSA on the same compound, same month — both cleared 98.0%

Suspicious Cold Box ×7 Sourced ×2 Receipts ×2

Numbers in the title, numbers in the post. TFC vs SSA on the same compound, same month — both cleared 98.0%.

Figures up front so nobody has to dig: 98.0%.

Sent one vial to VendorInvestigate and PeptideMeter. 97.8% and a claimed 97.0%. Cost me more than the vial and I would do it again.

Started keeping a folder of every certificate I have been sent. The ones that itemise impurities are a small group and they are mostly the same suppliers.

Ask me anything specific. Anything general I will probably get wrong.

8,140 up / 834 down91% upvoted48 commentsid 1y1pgo15 Mar 2024
Suppliers named in this thread
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48 comments

8 in this archive, depth 2

best — the order this archive was captured in

u/tidy_vialdrawer_watch281k points·2 years ago·edited

Ask what the stability statement was written against — temperature, light, and whether it was the lyophilised powder or the reconstituted solution. Those are three different claims.

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u/marisol_frisk340 points·2 years ago

Right, and net peptide content is where the counterion and the water go. Both numbers can be honest and very different.

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u/ahmed_rasmussen641 points·2 years ago

LC-MS gives you identity; UV purity gives you a relative quantity. A document with the first and not the second, or the reverse, is answering half the question.

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u/zeynep_zielinskiOP287 points·2 years ago

My first certificate had a compound name, a percentage and a logo. I thought that was normal. It is not, and now I know what to ask for.

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u/zaid_balogun561 points·2 years ago

The document I was worried about turned out to be a re-issue with a later creation date. Asked, got the original, entirely ordinary.

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u/zeynep_zielinskiOP401 points·2 years ago

Does the lot on the vial match the lot on the page?

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u/nadia_fonseca297 points·2 years ago

Yes — and the reason to ask for the whole page is that the interesting parts are the ones people crop out to fit a screenshot.

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u/zeynep_zielinskiOP429 points·2 years ago

That is the catalogue certificate rather than the batch-specific one. Same layout, completely different status.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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