genuine question about method development that I am slightly embarrassed to ask
genuine question about method development that I am slightly embarrassed to ask. Searched first, found three threads that contradict each other, hence the post. Resolution between two peaks depends on retention, selectivity and efficiency. A shallower gradient buys retention and usually resolution, at the cost of…
A shoulder that does not baseline-resolve cannot be quantified honestly. You can report it as an unresolved shoulder, which is useful information, or you can develop the method until it resolves.
Yes — system suitability first. Without it the number is an assertion about the instrument, not the sample.
Gave the same trace to two people I trust and got two integrations about a point apart. That was the most educational afternoon I have had here.
integration decisions move the number more than the sample does
ghost peak, check the plumbing first, it is always the plumbing
This. A shoulder that does not baseline-resolve is a question, not a quantity.
LC-MS for identity, UV for relative quantity