[Meta] proposal — a flair for gradient posts
Putting this to the board: proposal — a flair for gradient posts. A shoulder that does not baseline-resolve cannot be quantified honestly. You can report it as an unresolved shoulder, which is useful information, or you can develop the method until it resolves. System suitability — repeat injections, tailing factor,…
Yes. Retention time is a hypothesis about identity. Mass is the answer.
Resolution between two peaks depends on retention, selectivity and efficiency. A shallower gradient buys retention and usually resolution, at the cost of peak width and run time.
axis labels or the trace is decoration
LC-MS for identity, UV for relative quantity
Bought a second-hand instrument and learned more in six months of fixing it than in three years of reading traces.
retention time alone is not identity
Do you have the mass, or only the UV trace?
Do you have the mass, or only the UV trace?
Disagreeing with this bit: that spread is ordinary inter-lab variance, not a disagreement about the material.
What wavelength, and what was the gradient?