how much of what we believe about HPLC actually comes from LC-MS threads
how much of what we believe about HPLC actually comes from LC-MS threads. Searched first, found three threads that contradict each other, hence the post. Chased a ghost peak for three weeks. It was the plumbing. It is always the plumbing. A shoulder that does not baseline-resolve cannot be quantified honestly. You…
Left up. The integration disagreement in this thread is the most useful thing on the board this week.
That is area percent, not mass percent. The trace cannot give you the second one.
Bought a second-hand instrument and learned more in six months of fixing it than in three years of reading traces.
a blank injection between samples costs four minutes and settles most arguments
Correction: that is a tailing factor, not a plate count. Different diagnostics for different problems.