[Question] integration — what am I missing here
integration — what am I missing here — that is what I am asking, and I have already read the wiki twice. Mass spectrometry answers identity. UV purity answers relative quantity under the run conditions. A document with one and not the other is answering half the question, and the half it answers should be stated.…
Yes. Retention time is a hypothesis about identity. Mass is the answer.
Correction: that is a tailing factor, not a plate count. Different diagnostics for different problems.
What did system suitability look like on that sequence?
LC-MS for identity, UV for relative quantity
report the method or do not report the number
a blank injection between samples costs four minutes and settles most arguments
Bought a second-hand instrument and learned more in six months of fixing it than in three years of reading traces.
Same view — reporting the gradient is what makes a result checkable rather than merely stated.