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c/hplc·submitted 9 months ago by u/mariam_cabrera

does gradient actually matter or is it forum lore at this point

Discussionbranch of 7 comments

Trying to get a straight answer on this: does gradient actually matter or is it forum lore at this point. A shoulder that does not baseline-resolve cannot be quantified honestly. You can report it as an unresolved shoulder, which is useful information, or you can develop the method until it resolves. System…

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7 comments, started 9 months ago
u/crosspost_bot_no63 points·9 months ago

retention time alone is not identity

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u/honest_syringe_20250 points·9 months ago

a shoulder is not an impurity until you can resolve it

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u/rania_okonkwo0 points·9 months ago

a shoulder is not an impurity until you can resolve it

Agreed. And the corollary is that the method line on a certificate is not decoration, it is the number’s provenance.

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[deleted]1 point·9 months ago

[deleted]

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u/peak_area_peteMOD47 points·9 months ago

Left up. The integration disagreement in this thread is the most useful thing on the board this week.

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u/mariam_cabreraOP34 points·9 months ago

Small fix — 214nm, not 210. It matters for the comparison you are making with the other run.

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u/coa_janitormod · c/coa-26 points·9 months ago

axis labels or the trace is decoration

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