integration — 22 things I got wrong before I got it right
integration — 22 things I got wrong before I got it right. It is the sort of thing everyone half-believes and nobody writes down. Resolution between two peaks depends on retention, selectivity and efficiency. A shallower gradient buys retention and usually resolution, at the cost of peak width and run time. UV…
area percent is relative to what the detector saw and nothing else
area percent is relative to what the detector saw and nothing else
This is the whole methodological point of the board in one line.
Yes — system suitability first. Without it the number is an assertion about the instrument, not the sample.
What wavelength, and what was the gradient?
Correction: that is a tailing factor, not a plate count. Different diagnostics for different problems.
LC-MS for identity, UV for relative quantity